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  Indian J Med Microbiol
 

Figure 2: (a-c) A case of a vasculitis. (a) H and E staining, showing an upper dermal perivascular infiltrate (black arrows) (100×) (b) Positive staining with PAS around the same vessels as in a; the observed staining was not exclusively on neutrophils (purple staining; black arrows) (100×) (c) DIF, showing positive staining with fibrinogen FITC conjugated, against the same vessels (green staining; red arrow) (400×). The identity of the blood vessels was confirmed using CD31 immunohistochemistry. (d-f) A case of cutaneous lupus. (d) H and E staining shows damage to the basement membrane zone at the area of a hair follicle, and the inflammatory infiltrate (black arrow) (100×) (e) Shows a highlight of d, at the basement membrane zone (black arrow) (200×) (f) Shows DIF positive staining at the basement membrane zone of the sweat glands, using FITC conjugated anti-human IgM (green staining; white arrow). The nuclei of epidermal keratinocytes were counterstained with DAPI (light blue)

Figure 2: (a-c) A case of a vasculitis. (a) H and E staining, showing an upper dermal perivascular infiltrate (black arrows) (100×) (b) Positive staining with PAS around the same vessels as in a; the observed staining was not exclusively on neutrophils (purple staining; black arrows) (100×) (c) DIF, showing positive staining with fibrinogen FITC conjugated, against the same vessels (green staining; red arrow) (400×). The identity of the blood vessels was confirmed using CD31 immunohistochemistry. (d-f) A case of cutaneous lupus. (d) H and E staining shows damage to the basement membrane zone at the area of a hair follicle, and the inflammatory infiltrate (black arrow) (100×) (e) Shows a highlight of d, at the basement membrane zone (black arrow) (200×) (f) Shows DIF positive staining at the basement membrane zone of the sweat glands, using FITC conjugated anti-human IgM (green staining; white arrow). The nuclei of epidermal keratinocytes were counterstained with DAPI (light blue)