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  Indian J Med Microbiol
 

Figure 4: (a and b) Direct immunofluorescence. These show a representative case of discoid lupus, with a serrated deposit of complement/C3 along the basement membrane zone (green staining; white arrow); in (b) The nuclei of the cells are counterstained with 4',6-diamidino-2-phenylindole (light blue). In (c and d), a case of bullous pemphigoid, positive with fluorescein isothiocyanate conjugated immunoglobulin G (green staining at the basement membrane zone in a continuous pattern; yellow arrow); in (d) We utilize NaCl split skin with Ulex in red and 4',6-diamidino-2- phenylindole in blue. The stain, in this case, is present on both sides of the split, but primarily on the epidermal side (yellow arrow)

Figure 4: (a and b) Direct immunofluorescence. These show a representative case of discoid lupus, with a serrated deposit of complement/C3 along the basement membrane zone (green staining; white arrow); in (b) The nuclei of the cells are counterstained with 4',6-diamidino-2-phenylindole (light blue). In (c and d), a case of bullous pemphigoid, positive with fluorescein isothiocyanate conjugated immunoglobulin G (green staining at the basement membrane zone in a continuous pattern; yellow arrow); in (d) We utilize NaCl split skin with <i>Ulex</i> in red and 4',6-diamidino-2- phenylindole in blue. The stain, in this case, is present on both sides of the split, but primarily on the epidermal side (yellow arrow)