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  Indian J Med Microbiol
 

Figure 1: (a) An indirect immunofluorescence showing an intercellular stain between the queratinocytes (ICS) (white arrow) using monkey esophagus as substrate and fluorescein isothiocyanate conjugated anti-human immunoglobulin G antibodies. In (b), similar but using 4',6-diamidino-2-phenylindole to counterstain the nuclei of the cells (light blue) in red, antibody against anti-intercellular adhesion molecule 1/CD54 (vascular endothelial marker) conjugated with Alexa 555. In (c), an example of direct immunofluorescence using the patient skin from the same patient as in a and b; in d, same as c but using 4',6-diamidino-2-phenylindole to counterstain the nuclei

Figure 1: (a) An indirect immunofluorescence showing an intercellular stain between the queratinocytes (ICS) (white arrow) using monkey esophagus as substrate and fluorescein isothiocyanate conjugated anti-human immunoglobulin G antibodies. In (b), similar but using 4',6-diamidino-2-phenylindole to counterstain the nuclei of the cells (light blue) in red, antibody against anti-intercellular adhesion molecule 1/CD54 (vascular endothelial marker) conjugated with Alexa 555. In (c), an example of direct immunofluorescence using the patient skin from the same patient as in a and b; in d, same as c but using 4',6-diamidino-2-phenylindole to counterstain the nuclei