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  Indian J Med Microbiol
 

Figure 1A: AzA methylester standard run in GC. 1 mM of AzA methylester was esterified by BF3, extracted in ether and dried under nitrogen gas and then dissolved in hexane and injected in a Sigma-Supelco capillary column (50m X 0.25 mm, CP 6173); The column temperature was kept at 140 ° C for 0.5 min, and then gradually increased up to 225° C at the rate of 4° C per minute. The injector temperature was kept at 270 ° C and the detector (FID) temperature at 300°C. Figure 1B: AzA spiked in FAME standard. 1 part of 1 mM of AzA methylester was mixed with 4 parts of 1mg/ml FAME standards and injected in GC. The column temperature was kept at 140 ° C for 0.5 min, and then gradually increased up to 225° C at the rate of 4° C per minute. The injector temperature was kept at 270 ° C and the detector (FID) temperature at 300°C.

Figure 1A: AzA methylester standard run in GC. 1 mM of AzA methylester was esterified by BF3, extracted in ether and dried under nitrogen gas and then dissolved in hexane and injected in a Sigma-Supelco capillary column (50m X 0.25 mm, CP 6173); The column temperature was kept at 140 ° C for 0.5 min, and then gradually increased up to 225° C at the rate of 4° C per minute. The injector temperature was kept at 270 ° C and the detector (FID) temperature at 300°C. Figure 1B: AzA spiked in FAME standard. 1 part of 1 mM of AzA methylester was mixed with 4 parts of 1mg/ml FAME standards and injected in GC. The column temperature was kept at 140 ° C for 0.5 min, and then gradually increased up to 225° C at the rate of 4° C per minute. The injector temperature was kept at 270 ° C and the detector (FID) temperature at 300°C.