Antioxidant enzymes and lipid peroxidation in Type 2 diabetes mellitus patients with and without nephropathy

Manjulata Kumawat; Tarun Kumar Sharma; Ishwar Singh; Neelima Singh; Veena Singh Ghalaut; Satish Kumar Vardey; Vijay Shankar

doi:10.4103/1947-2714.109193

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ORIGINAL ARTICLE

Year : 2013 | Volume : 5 | Issue : 3 | Page : 213-219

Antioxidant enzymes and lipid peroxidation in Type 2 diabetes mellitus patients with and without nephropathy

Manjulata Kumawat¹, Tarun Kumar Sharma¹, Ishwar Singh², Neelima Singh³, Veena Singh Ghalaut¹, Satish Kumar Vardey⁴, Vijay Shankar¹
¹ Department of Biochemistry, Pandit Bhagwat Dayal Sharma University of Health Sciences, Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
² Department of Neurosurgery, Pandit Bhagwat Dayal Sharma University of Health Sciences, Post Graduate Institute of Medical Sciences, Rohtak, Haryana, India
³ Department of Biochemistry, G.R. Medical College, Gwalior, Madhya Pradesh, India
⁴ Department of Biochemistry, Swami Man Singh Medical College, Jaipur, Rajasthan, India

Correspondence Address:
Tarun Kumar Sharma
Department of Biochemistry, Pandit Bhagwat Dayal Sharma University of Health Sciences, Post Graduate Institute of Medical Sciences, Rohtak - 124 001, Haryana
India

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/1947-2714.109193

Background: Oxidative stress has been considered to be a pathogenic factor of diabetic complications including nephropathy. There are many controversies and limited studies regarding the antioxidant enzymes in diabetic nephropathy. Aim: This study was to evaluate the levels of antioxidant enzymes and lipid peroxidation in Type-2 Diabetes Mellitus (DM) patients with and without nephropathy. Materials and Methods: The study included 90 age and sex matched subjects. Blood samples of all subjects were analyzed for all biochemical and oxidative stress parameters. Results: The malondialdehyde (MDA) levels and catalase (CAT) activity were significantly increased and reduced glutathione (GSH) levels and activities of glutathione peroxidase (GPx) and glutathione reductase (GR) were significantly decreased in Type-2 DM with and without nephropathy as compared to controls and also in Type-2 DM with nephropathy as compared to Type-2 DM without nephropathy. There were an excellent positive correlation of glycohemoglobin (HbA1c) with MDA and a good negative correlation of GPx with GSH in controls. There were positive correlations of GR, CAT, and superoxide dismutase (SOD) with MDA in Type-2 diabetes patients with nephropathy. Conclusions: Intensity of oxidative stress in Type-2 diabetic patients with nephropathy is greater when compared with Type-2 diabetic patients without nephropathy as compared to the controls.

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