| ORIGINAL ARTICLE |
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| Year : 2013 | Volume
: 5
| Issue : 2 | Page : 95-101 |
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Genetic diversity of Plasmodium falciparum field isolates in central Sudan inferred by PCR genotyping of Merozoite surface protein 1 and 2
Muzamil M Abdel Hamid1, Sara B Mohammed2, Ibrahim M El Hassan3
1 Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan
2 Faculty of Veterinary Medicine, Sudan University of Science and Technology, Sudan
3 Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan; Faculty of Medicine, Jazan University, Saudi Arabia
Correspondence Address:
Muzamil M Abdel Hamid
Institute of Endemic Diseases, University of Khartoum, P.O. Box 102, Khartoum, Sudan
 Source of Support: This work received financial support from DAAD, Germany., Conflict of Interest: None  | Check |
DOI: 10.4103/1947-2714.107524
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Background : Characterization of Plasmodium falciparum diversity is commonly achieved by amplification of the polymorphic regions of the merozoite surface proteins 1 (MSP1) and 2 (MSP2) genes. Aims : The present study aimed to determine the allelic variants distribution of MSP1 and MSP2 and multiplicity of infection in P. falciparum field isolates from Kosti, central Sudan, an area characterized by seasonal malaria transmission. Materials and Methods : Total 121 samples (N = 121) were collected during a cross-sectional survey between March and April 2003. DNA was extracted and MSP1 and MSP2 polymorphic loci were genotyped. Results : The total number of alleles identified in MSP1 block 2 was 11, while 16 alleles were observed in MSP2 block 3. In MSP1, RO33 was found to be the predominant allelic type, carried alone or in combination with MAD20 and K1 types, whereas FC27 family was the most prevalent in MSP2. Sixty two percent of isolates had multiple genotypes and the overall mean multiplicity of infection was 1.93 (CI 95% 1.66-2.20). Age correlated with parasite density ( P = 0.017). In addition, a positive correlation was observed between parasite densities and the number of alleles (P = 0.022). Conclusion : Genetic diversity in P. falciparum field isolates in central Sudan was high and consisted of multiple clones. |
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