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Year : 2012  |  Volume : 4  |  Issue : 12  |  Page : 651-655

Construction of pET-32 α (+) vector for protein expression and purification

Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada

Correspondence Address:
Ping-Chang Yang
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1947-2714.104318

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The construction of expression vector is a basic tool for biotechnology and production of desired proteins, this article summarized the construction of pET-32 α (+) vector techniques which are generally used in research laboratories. The procedures include that acquisition of the exogenous DNA fragment for construction of the vector, subcloning the DNA fragment into pET-32 α (+) expression vector, protein expression in Escherichia coli BL21 (DE3) and protein purification under native conditions in E. coli lysates.

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