Cytotoxic T lymphocytes mediate chronic inflammation of the nasal mucosa of patients with atypical allergic rhinitis
Shuqi Qiu1, 2, 3,*, Yun Du4, 5,*, Xiaobei Duan1, Xiaorui Geng1, Jianxiong Xie2, Han Gao1, 2, 3, Ping-Chang Yang4
1Shenzhen ENT Institute, 2Shenzhen Longgang Central Hospital, 3ENT Hospital, Shenzhen, China.
4Department of Pathology & Molecular Medicine, McMaster University, Hamilton, Canada.
5Department of Pathology, Hebei Medical University, Shijiazhuang, China.
Citation: Qiu S, Du Y, Duan X, Geng X, Xie J, Gao H, Yang P-C. Cytotoxic T lymphocytes mediate chronic inflammation of the nasal mucosa of patients with atypical allergic rhinitis. North Am J Med Sci 2011; 3: 378-383.
* Authors equally contributed to this work, and are the joined first authors.
Background: The prevalence of chronic rhinitis is increasing rapidly; its pathogenesis is to be further understood; immune inflammation is one of the possible causative factors. Antigen specific CD8+ T cells play a critical role in the induction of chronic inflammation. Aims: This study aimed to investigate the role of antigen specific CD8+ T cells in the pathogenesis of chronic atypical allergic rhinitis. Material and Methods: Nasal mucosal epithelial surface scratching samples were obtained from patients with chronic obstruction atypical allergic rhinitis. Exosomes were purified from the scratching samples and examined by immune gold electron microscopy. The effect of exosomes on modulating dendritic cell’s properties, the effect of exosome-pulsed dendritic cells on naïve T cell differentiation and the antigen specific CD8+ T cell activation were observed by cell culture models. Results: Exosomes purified from patients with chronic atypical allergic rhinitis carried microbial products, Staphylococcal enterotoxin B (SEB), and airborne antigen, Derp1. Dendritic cells pulsed by SEB/Derp1-carrying exosomes showed high levels of CD80, CD86 and the major histocompatibility class I (MHCI). Exosome-pulsed dendritic cells could induce the naïve CD3+ T cells to differentiate into CD8+ T cells. Upon the exposure to a specific antigen, the CD8+ T cells released granzyme B and perforin; more than 30% antigen specific CD8+ T cells proliferated. Conclusions: Antigen specific CD8+ T cells play an important role in the pathogenesis of chronic obstruction atypical allergic rhinitis.
Keywords: Rhinitis, CD8 T lymphocytes, Epithelium, Antigen specific response.
Dr. Shuqi Qiu, Shenzhen ENT Institute, Shenzhen Longgang Central
Hospital, Shenzhen, Guangzhou, China.
Tel. /Fax: 0755 2658 6018, Email:firstname.lastname@example.org
or Dr. Ping-Chang Yang. Room T3303,