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Year : 2014  |  Volume : 6  |  Issue : 3  |  Page : 160

Western blot: Technique, theory and trouble shooting


Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada

Date of Web Publication11-Mar-2014

Correspondence Address:
Ping-Chang Yang
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON
Canada
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1947-2714.128482

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How to cite this article:
Liu ZQ, Mahmood T, Yang PC. Western blot: Technique, theory and trouble shooting. North Am J Med Sci 2014;6:160

How to cite this URL:
Liu ZQ, Mahmood T, Yang PC. Western blot: Technique, theory and trouble shooting. North Am J Med Sci [serial online] 2014 [cited 2017 Mar 30];6:160. Available from: http://www.najms.org/text.asp?2014/6/3/160/128482

Dear Editor,

We appreciate the comments [1] regarding our published article "Western blot: Technique, theory, and trouble-shooting". [2] For transferring of proteins to a membrane, the method of "wet transfer" in Western blotting is routinely utilized by laboratories and guided by the protocols online. [3] Heat may be produced during the transfer; according to the recommended protocols, placing the transfer apparatus on ice or placing it in the cold room is necessary. It should be considered that the purpose of 'Figure 7' in our article [2] is to show how to cool down the environmental temperature to 4°C by placing the apparatus on ice, which cannot be misunderstood as submerging the electroporator or the power leads in the water bath. In addition, we agree with the proposal that safe laboratory practice should always be observed to avoid chemical or electrical hazards in operating the Western blot transferring systems.

 
  References Top

1.Gibbons J. Western blot: Protein transfer overview. N Am J Med Sci 2014;6:158-9.  Back to cited text no. 1
    
2.Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting. N Am J Med Sci 2012;4:429-34.  Back to cited text no. 2
    
3.Protocol Online. (Accessed December 25, 2013, at www.protocolonline.org/prot/Molecular_Biology/Protein/Western_Blotting ).  Back to cited text no. 3
    



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